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30 Mar 2012 Adipocyte differentiation of 3T3-L1 cells was induced by using standard hormonal cocktail. In brief, at two Cells were transfected with 10 nmol/L siRNA using Lipofectamine RNAiMAX, according to the manufacturer's protocol (Invitrogen). The three Transmission electron microscopy (TEM). Three days
10 Oct 2016 Quantitatively, however, it remained comparable or lower than that for commercial transfection reagents, Lipofectamine RNAiMax (Lipo) and . In accord with this, transmission electron microscopy (TEM) revealed 2040 nm aggregates for HAL and marginally larger nanoparticles (?55 nm) with a
MAN0007825 Rev.1.0. Lipofectamine RNAiMAX Reagent. Protocol Outline. A. Plate cells so they will be 60-80% confluent at the time of transfection. B. Prepare RNA-lipid complexes. C. Add RNA-lipid complexes to cells. Lipofectamine RNAiMAX Transfection Protocol. See page 2 to view a typical RNAiMAX transfection
26 Jan 2013 The stearic acid was coupled manually to the N-terminus of the peptide overnight, at room temperature with 5 eq. stearic acid. For the synthesis . For transmission electron microscopy (TEM) studies the nanogold-labeled 705 pLuc splice-correcting oligonucleotides (NG-SCO) were used. The thiol group at
standard reversed-phase liquid chromatography (e.g. very high or very low hydrophobicity). Example 1. Two interacting .. RNAiMAX transfection reagent (Invitrogen) according to the manufacturer's instructions. The Luciferase GL2 Duplex . Tecnai 20 transmission electron microscope (TEM). Western Blots. For Western
22 Nov 2012 Transmission electron microscopy (TEM) examination of the ultrastructural features of untreated U937 cells revealed well-preserved and morphologically .. with siRNA corresponding to human JNK1 or JNK2 and with scr-siRNA, using Lipofectamine RNAiMAX according to the manufacturer's guidelines.
Lipofectamine RNAiMAX Transfection Reagent - Delivery of siRNA. View the video to see the steps needed to perform siRNA transfection with Lipofectamine RNAiMAX reagent. When it comes to achieving effective gene knockdown, Lipofectamine RNAiMAX Transfection Reagent easily
21 Aug 2014 Mutations in WT1 are typically heterozygous and are usually germline or de novo, although occasional parent-to-child transmission has been reported. WT1 encodes a zinc finger .. Next, cells were transfected with control or WT1 small interfering RNAs using RNAiMax transfection reagent. Cells were then
Lipofectamine RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically
2 Oct 2014 The thin sections were double stained (uranyl acetate and lead citrate), examined in a Hitachi H-7650 transmission electron microscope (Hitachi High . pool control siRNAs (Thermo-Fisher Scientific/Dharmacon, Pittsburgh, PA) using Lipofectamine RNAiMAX/Opti-MEM (Life Technologies) for up to 144 h.